Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT

產(chǎn)品型號(hào)：
產(chǎn)品時(shí)間：2024-08-12
簡(jiǎn)要描述：慧穎生物專業(yè)供應(yīng)Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT，常備現(xiàn)貨，*。主營(yíng)Life Invitrogen產(chǎn)品，囊括TRlzol ®Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。

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產(chǎn)品名稱：Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT

貨號(hào)：K494500

規(guī) 格：20 RXNS

分類：RNAi 載體

品牌：Invitrogen

國(guó)產(chǎn)/進(jìn)口：進(jìn) 口

儲(chǔ)存溫度：-20°C

特惠價(jià)格：致電

庫(kù)存狀態(tài)：現(xiàn) 貨

銷售商：上?；鄯f生物科技有限公司

慧穎生物專業(yè)供應(yīng)Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT，常備現(xiàn)貨，*。主營(yíng)Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。

Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT描述：

The BLOCK-iT? U6 RNAi Entry Vector provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi), a preferred technique for analyzing gene downregulation. An easy cloning process places an ~50-bp oligonucleotide DNA immediay following a U6 pol III type promoter (Figure 1). Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA (siRNA) that will generate the RNAi effect.
Delivery of the U6 RNAi Cassette
Once cloning is complete, the U6 Entry Vector is ready to be used immediay in transient transfections using a reagent such as Lipofectamine? 2000. This makes this system ideal for initial shRNA screenings in many mammalian cell types. As an alternative in hard-to-transfect or non-dividing cell types, or to deliver to animal model systems, the RNAi cassette can easily be recombined into a BLOCK-iT? Viral RNAi vector. For stable shRNA lentiviral delivery and expression, recombine the BLOCK-iT? U6 Entry Vector with the pLenti6/BLOCK-iT? RNAi Vector. For transient delivery to challenging cell types using adenoviral transduction, recombine with the pAd/BLOCK-iT? RNAi Vector.

Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT同類產(chǎn)品主要有:

貨號(hào)	名稱	規(guī)格
18064-014	SuperScript? II Reverse Transcriptase	10,000U
18080-044	SuperScript? III Reverse Transcriptase	10,000U
18418-012	Oligo(dT)12-18 Primer	25ug
18418-020	Oligo(dT)20 Primer	50ul(50uM)
10777-019	RNaseOUTTM Ribonuclease Inhibitor	5,000U
18080-051	SuperScript? III First-Strand	50 rxn
18080-051	Synthesis System for RT-PCR	50 rxn
11668-027	Lipofectamine TM 2000 Reagent	0.75ml
11668-019	Lipofectamine TM 2000 Reagent	1.5ml
13778-150	Lipofectamine RNAiMax	1.5ml
13778-075	Lipofectamine RNAiMax	0.75ml
15596-026	TRlzol ?Reagent	100ml
15596-018	TRlzol ?Reagent	200ml
10296028	TRIZOL LS Reagent 200 ml（長(zhǎng)方形）	200ml
10296010	TRIZOL LS Reagent 100ml（長(zhǎng)方形）	100ml
11789020	BP CLONASE II ENZYME MIX	20 REACTIONS
11791020	LR CLONASE II ENZYME MIX	20 REACTIONS
12328040	AMV	100 REACTIONS
12328040	(CLON AMV FIRST STR SYNTH KIT)	100 REACTIONS
28025-013	M-MLV Reverse Transcriptase	40,000U
17504-044	B-27（黃顏色）	10ml
12587-010	B-27（黃顏色）不含維生素A	10ml
15090-046	2.5% Trypsin (10X), no Phenol Red	100ml
15050-065	0.25% Trypsin(1X),Phenol Red	100ml
15050-057	0.25% Trypsin(1X),Phenol Red	500ml
15140-122	Pen Strep	100ml
15140-163	Pen Strep	20X100ml
25200-056	0.25% Trypsin-EDTA(1X,Phenol Red)	100ml
25200-114	0.25% Trypsin-EDTA(1X,Phenol Red)	20X100ml
25300-054	0.05% Trypsin(1X),Phenol Red:	100ml
25300-120	0.05% Trypsin(1X),Phenol Red:	20X100ml
15400-054	0.5% Trypsin(10X),:	100ml
25030-081	L-Glutamine 200mM	100ml
25030-164	L-Glutamine 200mM	20X100ml
12328019	CLONED AMV RT, 750U	TRANSCRIPTASE
12328032	CLONED AMV FIRST STRA SYN KIT	25 REACTIONS
10966-018	Platinum? Taq DNA聚合酶	100 rxns
10966-026		250 rxns
10966-034		500 rxns
K210007	PURELINK PLASMID MAXI 25	25 REACTIONS
15224041	T4 DNA LIGASE HI CONC.	250 U
C737303	ONE SHOT STBL3 COMP E COLI	ONE SHOT STBL3 20 RXN
12033015	ELECTROMAX DH10B T1 CELLS	0.5 ML (5 X 0.1 ML)
A10460	ONE SHOT CCDB SURVIVAL 2-T1R	10 REACTIONS
LC5925	SEEBLUE PLUS2	500UL
11708039	PLATINUM PFX DNA POLYMERASE	500 REACTIONS #95514- #52806
12344024	ACCUPRIME PFX DNA POLYMERASE	200 REACTIONS
12346086	ACCUPRIME TAQ HIFI	200 RXNS
11495017	PCRX ENHANCER SYSTEM	250 RXN 52395
450008	TOPO XL PCR CLONING KIT	10 RXN
450008	TOPO XL PCR CLONING KIT	10 RXN
MECOV50	METHYLCODE BISULFITE CONV KIT	50 REACTIONS
12935300	STEALTH RNAI NEG CTL MED GC	250 μl
2013	FLUORESCENT OLIGOMER, 20UM	250 UNITS
2013	FLUORESCENT OLIGOMER, 20UM	250 UNITS
K159303	KIT,FAST TRACK	18 RXN
11917020	SS DBL STRND CDNA SYN KIT	50 RXNS
LC5677	MARK12 W RANGE MW STD,1ML	1 ML

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Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT

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