Invitrogen 18080-044 SuperScript® III Reverse

產品型號：
產品時間：2024-08-12
簡要描述：慧穎生物專業(yè)供應Invitrogen 18080-044 SuperScript® III Reverse Transcriptase，10,000U，*，常備現(xiàn)貨，-20°C儲存，咨詢！

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產品簡介

Invitrogen 18080-044 SuperScript? III Reverse Transcriptase 簡介：

產品名稱：SuperScript? III Reverse Transcriptase

中文名稱：反轉錄酶III

品牌：Invitrogen

貨號：18080-044

規(guī)格：10,000 units

市場價格：4245元

儲存溫度：-20°C

庫存狀態(tài)：現(xiàn)貨

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Invitrogen 18080-044 SuperScript? III Reverse Transcriptase 英文描述：

SuperScript? III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript? II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)⁺ RNA sample. Like SuperScript? II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript? III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

? Thermostability – Half life of 220 minutes at 50°C
? Yield – Reduced RNase H activity for more full-length cDNA
? Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
? Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3′ and 5′ RACE

Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.

Unit Definition: One unit of SuperScript? III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)_12-18 as a template?primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl₂, 1 mM DTT, 0.5 mM [³H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)_12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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